Read FLUOstarOPTIMAbrochure.pdf text version FLUOstar OPTIMA An exceptional combination of Flexibility and Performance Flexibility The FLUOstar OPTIMA is a versatile microplate reader offering four separate reading modes with integrated resonance energy transfer detection capabilities (FRET, BRET). Fluorescence Intensity Time-Resolved Fluorescence Luminescence (Flash and Glow) Absorbance (low UV to Vis) The FLUOstar OPTIMA reads up to 384-well plates in all detection modes and provides 1536-well plate capability for fluorescence intensity measurements.
Kinetic events can be initiated with the onboard reagent injectors and due to the individually adjustable delivery volumes for each well, dilution schemes and concentration ranges can be created throughout the plate. Top and bottom plate reading, precise temperature control up to 45°C (optional 60°C), well scanning, multimode shaking capabilities and gas vent connection enhance the assay flexibility of the FLUOstar OPTIMA. Filter-Based Detection Filters offer precise and superior performance for both sensitivity and selectivity for fluorescence and Modular Multidetection luminescence assays. In particular, filters offer high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent blockage of undesired wavelengths, and fast wavelength switching.
FLUOstar OPTIMA - The optimal combination of performance and flexibility for all of your R&D applications.Whether you need sensitivity, flexibility or extensive kinetic capabilities, the FLUOstar OPTIMA from BMG LABTECH is the optimal solution for academic and pharmaceutical research. The iNANO House, Gustav Wieds Vej 14, Aarhus University. DK-8000 Aarhus C. (Casa Software Ltd, UK) software. Three separately. Nm and emission of 590 nm using a plate reader (FLUOstar OPTIMA, BMG Labtech GmbH.
This is ideal for multi-excitation and multi-emission applications. Filters are also the technically preferred and most cost efficient technology and BMG LABTECH offers a wide range of high-performance assay specific filters from UV to NIR with various bandwidths. Microplate Reader with 4 Detection Modes Sensitivity, flexibility and extensive kinetic capabilities the FLUOstar OPTIMA is the optimal solution for academic research. High-performance optical filters from UV to NIR Advanced Reagent Injection Two precise onboard injectors with an exceptional low dead volume allow simultaneous reagent injection and detection. Users can adjust all parameters, such as plate shaking, injection speed, timing, and the number of injections per well. Delivery volumes are adjustable for each well, so dilution schemes and concentration gradients can be automatically produced across the microplate.
![Fluostar Fluostar](/uploads/1/2/5/3/125396580/829188583.png)
The injectors are readily accessible and are housed within the instrument to safeguard any light sensitive reagents. Well Scanning and Orbital Averaging In well scanning mode, the FLUOstar OPTIMA can easily deal with nonhomogeneous samples such as adherent cells by taking multiple measurements in each well with up to 30 x 30 data points. The software displays each scan point graphically and creates a map for each well. Another way to measure nonhomogeneous well content is BMG LABTECH's unique orbital averaging feature. This allows several measurements over a defined orbit, to collect data and calculate an average for each well. Injection Optimized for Kinetic Reactions Kinetic assays, such as Ca2+ fluorescence measurements or flash luciferase luminescence, can be easily measured with a high degree of precision and reproducibility.
The precision syringe injectors have direct access to the plate measurement position, thereby ensuring that you do not miss any experimental data. The control software puts you in command over injection timing and pump settings such as injection speed, delivery volumes and the number of reagent injections per well. Kinetic data can be collected at a rapid 50 reading points per second, or as slow as one point every 2 ½ hours. CELL TRACKER SCAN IDs: clear well scan 96 A B C D E F G H Well: Untreated siControl TNFR1 TNF ng/mL 0 0.001 0.01 0.1 1.0 10 Blank No HUVEC H1.
1 10 Blank Blank Display intervals: 1 Well scanning view of cell tracker green labelled HL60 adhesion to TNF stimulated HUVEC (human umbilical vein endothelial cells). Blank wells contain unlabelled HUVECs in media, the 'no HUVEC' wells contain labelled HL60 to check adhesion to well surface. Incubation, Shaking and Gas Vent All cell-based assays require the correct environment to be properly measured. The FLUOstar OPTIMA has a gas. Fast filter switching and reagent injection vent, incubation, and multimode shaking capabilities. A gas vent can be used to purge the microplate chamber with different gases. Inert gases such as nitrogen can be used to measure growth in anaerobic conditions, or a mixture of High-Performance Luminescence The FLUOstar OPTIMA has been designed with a dedicated luminescence measurement system so you do not have to make a choice between a microplate fluorometer and a stand-alone luminometer.
The FLUOstar OPTIMA offers exceptional luminescence performance in a single instrument package that easily fulfilled Promega's stringent DLReadyTM (Dual Luciferase validation) criteria in 96- and 384-well plate formats. O2/CO2 can be used to measure mammalian tissue cells. The built-in incubator evenly heats the microplate chamber up to 45°C (optional 60°C), thereby allowing the perfect temperature to be used for all cellular growth assays. The FLUOstar OPTIMA can also be used for protein denaturation studies by incrementally increasing the temperature in the reader.
Multimode shaking - linear, orbital, and double orbital permit flexibility when designing assays. Linear shaking allows for the fastest and harshest shaking conditions for cells that tend to clump. Double orbital shaking allows for the slowest and mildest conditions for sensitive cells. While orbital shaking is a cross in between the two. Control and MARS Data Analysis Software The FLUOstar OPTIMA software package provides an extensive range of possibilities for both test protocol definitions and data analysis. It is fully compliant with FDA regulation 21 CFR Part 11.
The Reader Control part of the software defines instrument parameters and test protocols, and MARS offers various tools for data analysis. Well organized, versatile, easy to use and powerful are just a few of the ways the MARS Data Analysis Software package is described by users. MARS provides several options to display data in a clear and concise format.
Incubate, shake and detect any plate format. The Data Analysis Software MARS allows the user to display and process data using predefined templates. One example is the automatic calculation of enzyme kinetic parameters (Vmax and Km) with a variety of fits based on Michaelis- Stacker and Robot Compatibility For medium level throughput, BMG LABTECH offers a Stacker that can be used with the FLUOstar OPTIMA. The Stacker is an ideal solution for mid-throughput labs that wish to have the small footprint of an automated plate feeder along with the simplicity and reliability the Stacker offers. It provides loading, unloading, restacking and a continuous load feature of up to 50 microplates. All BMG LABTECH microplate readers have similar x-y dimensions and plate in/out locations. This minimizes the costs for customized automation solutions.
BMG LABTECH has cultivated collaborations with various robotics companies over the years and thus many proven robotic integrations are available. Menten or Lineweaver-Burk equations. The software is also capable of creating standard curves based on the following curve fitting algorithms to calculate values such as EC50, IC50 and r2: Linear regression fit 4 parameter fit Point to point fit Segmental regression fit Cubic spline fit 2nd and 3rd polynomial fit Enzyme kinetic (e.g. Michaelis-Menten).automated plate handling with Stacker The MARS Data Analysis Software provides sophisticated tools for automated data reduction.